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10.2337/db23-127-OR 77.Wilson JM, Nikooienejad A, Robins DA, et al
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Real-time quantitative polymerase chain reaction (RT-qPCR) The samples were added into Trizol for cracking, then chloroform was added for centrifugation to obtain the upper liquid phase, and isopropyl alcohol was added for centrifugation to obtain the precipitate
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