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GIP, blood GIP level gip In vivo measurements of β-cell glucose sensitivity are made by calculating the slope of the insulin secretion rate versus the blood glucose level during a graded glucose infusion study, which gradually raises blood glucose levels, typically from 5 to 12 mmol/L (Fig
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Islets were obtained from normoglycemic donors, according to the local ethics rules (including next-of-kin consent) and isolation techniques, and cultured in RPMI supplemented with 5.5 mM d-glucose, 10% FCS, 100 U penicillin, 100 μg streptomycin, and 0.25 μg μl −1 fungizone (37 °C, 5% CO 2 ) 28